LOCUS       40924_34131        3975 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pPBT 1.5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 3975)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva mutabilis RbcS promoter
                     /note="Ulva mutabilis RbcS promoter"
                     /regulatory_class="promoter"
     CDS             547..918
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      922..1310
                     /label=Ulva mutabilis RbcS terminator
                     /note="Ulva mutabilis RbcS terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(1338..1354)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1828..1932
                     /label=AmpR promoter
     CDS             1933..2790
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2964..3552
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3840..3861
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3876..3906
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3914..3930
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3938..3954
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gctatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacatgg ccaagatcac cagcgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
      601 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
      661 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
      721 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
      781 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
      841 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
      901 gtggccgagg agcaggacta agcagtcagg cagagtcgtt gttgggtgta tggttgcaca
      961 aagctagacc tcgagctggt cggttagaaa gagatgcttg tgcgactgga tgtaacaggc
     1021 acaatccgag gaaaatgcaa cagaaacgtt atgatgtaac atctgagcac agtcaataaa
     1081 attgcatgtt gtatgccgca cacaaaagtg ttgttaaaca tgcgatgtgg ttgtcaaatc
     1141 attattcgca caaccatgga ccgaacctct ggcacgctgc atacgctgta ttcattcaca
     1201 tcatgttttc tacttgtcac atgctctgca ccaagtaact ccgattagtt aggttcgcac
     1261 atgtcatcgg agtggaccac ttcgaatcat ttggttcgaa gacaaaccgg ggaattcgac
     1321 ctgcagccaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1381 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1441 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     1501 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     1561 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     1621 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     1681 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     1741 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     1801 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1861 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1921 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1981 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     2041 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     2101 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     2161 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     2221 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     2281 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     2341 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     2401 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     2461 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     2521 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2581 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2641 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2701 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2761 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2821 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3721 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     3781 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     3841 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     3901 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     3961 tacgaattcc catgg
//
